When meaning "nevertheless", add a comma after "still" at the start of a sentence or clause. When using "still" as a regular adverb, to modify a single word, do not use commas to separate "still" from the verb or adjective it describes.

At the beginning of a sentence, transition words such as "of course" and "certainly" are often followed by a comma. Some style guides, however, consider that this comma is not always necessary.

At the start of a sentence, add a comma after the conjunctive adverb "regardless" or the discourse marker "anyway" to introduce a sentence or clause.

At the start of a sentence, using a comma after "meanwhile" or "in the meantime" is optional. Commas are also optional in mid-sentence, but we should use them to signal a strong interruption or include nonessential information.

Add commas after "first", "second", "third", etc. when introducing a series of items. But a comma is generally unnecessary (and sometimes incorrect) when using "first" as an adverb of time, an adjective, or a noun.

At the beginning of a sentence, you do not need to add a comma after "yesterday", "tomorrow", "today", "last week", "soon", etc.

Do not use a comma after "now" at the start of a sentence to describe when something happens.

At the beginning of a sentence, use a comma after the conjunctive adverb "instead" to indicate that it modifies the whole sentence that follows.

At the beginning of a sentence, we add a comma after the conjunctive adverbs "afterward", "then", "and then", "subsequently", "after that", or "later" to indicate that it modifies the complete sentence that follows.